Nanostructures Using Self-organisation

In the field of the functionalisation of surfaces for biological applications, we have developed Self-Assembly monolayers (SAMs) of isocyanates on oxidized silicon wafers (1,0,0) by reaction of isocyanatodecyltrichlorosilane with the Si-OH groups of the surface. Excellent reactivity of the isocyanate monolayer with different nucleophiles was demonstrated. In particular, the semicarbazide group was obtained by reaction with Boc-hydrazine and deprotection of the tert-butyl carbamate. Alternatively, SAMs of semicarbazide groups were obtained by direct hydrosilylation of silicon wafers (1,1,1). The SAMs of semicarbazide group were very efficient for the grafting of functionalized peptides in mild conditions (figure 1). Biomolecular recognition with proteins or antibodies was then performed.